华北农学报2011，Vol.26Issue(3)：56-59,4.
人热休克蛋白10和鼠热休克蛋白10的克隆、序列分析及其原核表达
Cloning, Sequencing and Prokaryotic Expression of Homo Sapiens Heat Shock 10 kDa Protein and Mus Heat Shock 10 kDa Protein
摘要
Abstract
RT-PCR was used to study the expression of heat-shock 10 kDa protein. The results showed that there were two different HSP transcriptons of varied sizes in Homo liver cancer SMMC-7721 and Mus NSO. One HSP cDNA was composed of 312 bp (named HSPE1-1) and held 99% homology with HSPE1 mRNA reported ( NM-002157). The other HSP gene cDNA was consisted of 313 bp ( named Hspel-1) and had 97% homology with Hspel mRNA reported (NM-008303). The homology between HSPE1-1 and Hspel-1 was 92% in nucleotide sequence and 97% in amino acid sequence. Prokaryotic expression plasmids pET-28a-HSPEl-l and pET-28a-Hspel-1 for producing HSPE1 and Hspel proteins were constructed successfully. Its molecular weight was 14 kDa. Anti-His6 monoclonal antibody was used to demonstrate the identity of the proteins in Western Blot. The corresponding proteins were obtained after purification by Ni-NTA chromatography. The purified corresponding proteins would be helpfulin further studies of their molecular structure, biological function and clinical application.关键词
热休克蛋白/人热休克蛋白1/鼠热休克蛋白1/基因克隆/原核表达Key words
Heat-shock 10 kDa protein (Hsp10)/ Homo sapiens heat shock 10 kDa protein 1 ( chaperonin10) (HSPE1) / Mus musculus heat shock protein 1 (chaperonin 10) (Hspel) / Gene cloning/ Protein expression分类
生物科学引用本文复制引用
王丽,杜晓明,王林林,史西保,藤蔓,李功权,权凯,郭军庆,张改平..人热休克蛋白10和鼠热休克蛋白10的克隆、序列分析及其原核表达[J].华北农学报,2011,26(3):56-59,4.基金项目
河南省科技攻关项目(0823004532084) （0823004532084）
