华北农学报2011，Vol.26Issue(3)：60-63,4.
氨苄青霉素单克隆抗体及其与青霉素类抗生素交叉反应性
Anti-ampicillin Monoclonal Antibodies and Their Cross-reactivity to Penicillin Antibiotics
摘要
Abstract
Ampicillin ( Amp) was coupled to carrier proteins of bovine serum albumin ( BSA ) and ovalbumin (OVA) to synthetize immunogen BSA-Amp and detection antigen OVA-Amp. Four hybridoma cell lines secreting monoclonal antibodies (mAbs) specific for Amp were established by immunizing BALB/c mice with BSA-Amp u-sing hybridoma technology. The antibody titers of mAb ascites (1G7, 2A11, 2D10 and 3F6) were determined as 1:2.56×107, 1:1.28×107, 1 :5.12 × 106 and 1:5.12 × 106 in ELISA, and their half maximal inhibitory concentration (IC50) of Amp were 10.22, 3.58, 4.03,4.95 ng/mL in the inhibitive ELISA, respectively. The Amp mAbs showed significant cross-reactivity to the penicillin antibiotics of carbenicillin (1G7, 2A11, 2D10 and 3F6 ) , penicillin G (1G7 and 3F6) , and amoxicillin (1G7) , whereas no obvious cross-reactivity to dicloxacillin or clox-acillin was found.关键词
氨苄青霉素/单克隆抗体/交叉反应Key words
Ampicillin/ Monoclonal antibody/ Cross-reactivity分类
生物科学引用本文复制引用
李青梅,刘庆堂,王寅彪,柴书军,王自良,郭军庆,职爱民,孟红丽,张改平..氨苄青霉素单克隆抗体及其与青霉素类抗生素交叉反应性[J].华北农学报,2011,26(3):60-63,4.基金项目
"十一五"国家科技支撑计划(2006BAK02A21) （2006BAK02A21）
