华北农学报2011，Vol.26Issue(3)：68-71,4.
感染PRRSV Nsp2基因部分缺失变异株的仔猪抗体变化规律
The Changed Rule of Infected Piglets by a Mutant of Porcine Reproductive and Respiratory Syndrome Virus with Nsp2 Gene Deletion
摘要
Abstract
In order to understand the pathogenicity of PRRSV about Nsp2 gene deletion mutant, The PRRS negative healthy piglets of 20 days-old were inoculated by intranasal with a Mutant of PRRSV Hn-2 ( GenBank; FJ237419)and Hn-4 (GenBank; FJ237421) strains respectively, which delete 3 base pairs and 89 base pairs in the Nsp2 gene respectively, while healthy piglets were used for control, the sera were separated aseptically on the 0,7, 14,24,32,41,50,60,70 d respectively, using RT-PCR to detect the virus, using ELISA method and hnmunofluo-rescence inhibition test for detection of anti-PRRSV N protein antibody and neutralizing antibodies, variation of the virus and antibody were comparied in different periods of time. The results showed that the PRRSV could be detected in 7 -14 d after infected with the Mutant Hn-2, and Hn-4 strain, anti-PRRSV N protein antibodies could be detected on the 7th day and has maintained a high level, respectively, the low level of neutralizing antibodies could be detected respectively on 41 d and 50 d, and the neutralization antibody increased gradually. The test pigs were not of death, indicating that the mutant PRRSV with deleted Nsp2 gene does not necessarily lead to pig death.关键词
猪繁殖与呼吸综合征病毒/N蛋白/中和抗体/Nsp2基因Key words
PRRSV/N protein/ neutralization antibody/ Nsp2 gene分类
农业科技引用本文复制引用
张凤华,卢晓艳,徐红运,赵琳,刘玉松,范旭,夏平安,崔保安..感染PRRSV Nsp2基因部分缺失变异株的仔猪抗体变化规律 [J].华北农学报,2011,26(3):68-71,4.基金项目
河南省重点攻关项目(92102110018) （92102110018）
