华北农学报2011，Vol.26Issue(3)：95-99,5.
不同成熟培养时间对家猫卵母细胞皮质颗粒分布的影响
Effects of Duration on Oocyte Cytoplasm Maturation in vitro with CGs Staining
摘要
Abstract
In order to evaluate the effect of duration on oocyte cytoplasm maturation, the oocytes matured 24, 28, 32, 36 and 43 h were fixed in 3.7% paraformaldehyde and stained in FITC-LCA and PI, and the distribution of cortical granules (CGs) was observed to evaluate the cytoplasmic maturation. CCs were found throughout the oocyte cytoplasm. The CGFD didn't appear in oocytes of domestic cat. There were more CGs located in the cortex of the oocytes which were matured 32 h than the other groups. CGs decreased in oocytes matured 36 h and 43 h. The resultes showed that oocytes matured 32 h got ready to be sitimulated, and after 32 h the oocytes were degraded. Therefore, 32 h was the best maturation duration of domestic cat oocytes IVM.关键词
家猫/卵母细胞/皮质颗粒Key words
Domestic cat/Oocyte/Cortical granules分类
农业科技引用本文复制引用
王新庄,秦佳晨,任卫青,何俊丹,吕婧玉..不同成熟培养时间对家猫卵母细胞皮质颗粒分布的影响 [J].华北农学报,2011,26(3):95-99,5.基金项目
河南省基础与前沿技术研究计划(092300410081) （092300410081）
