华北农学报2011，Vol.26Issue(3)：195-198,4.
灵芝多糖对黄颡鱼免疫细胞活性的影响
Effects of Ganoderma lucidum Polysaccharides on the Activation of Immunological Cells of the Yellow Catfish (Pelteobagrus fulvidraco)
摘要
Abstract
In this test, 540 yellow catfish (Pelteobagrusfulvidraco) were selected, and fed with different levels of Ganoderma lucidum Polysaccharides (300, 600, 900, 1 200, 1 500 mg/kg diet, respectively) for 8 weeks. Then the head kidney macrophages and peripheral blood leukocytes of yellow catfish were separated by Percoll continuous density gradient centrifugation. NBT reduction, Griesse reagent coloration and MTT assay were respectively used to evaluate the respiratory burst activity of head kidney macrophages and proliferation of peripheral blood leukocytes , in order to discuss the effects of CLP on the activation of immunological cells of yellow catfish. The results showed that: the activity of oxygen respiratory burst activity of head kidney macrophages and the activity of proliferation of peripheral blood leukocytes of every levels of GLP group were extremely enhanced ( P <0. 01). Although the nitrogen respiratory burst activities of every level of GLP group were increased, only activities of 1 200 - 1 500 mg/kg GLP groups were obviously improved (P<0.01). As a result, Ganodeima lucidum polysaccharides could effectively enhance the activation of immunological cells of the Yellow catfish.关键词
黄颡鱼/灵芝多糖/免疫细胞活性/呼吸爆发/细胞增殖Key words
Yellow catfish ( Pelteobagrus fulvidraco) / Ganoderma lucidum polysaccharides/ Activation of immunological cells/ Respiratory burest activity/ Proliperation分类
农业科技引用本文复制引用
吴旋,白东清,杨广,宁博,张雪涛,孙立卓..灵芝多糖对黄颡鱼免疫细胞活性的影响 [J].华北农学报,2011,26(3):195-198,4.基金项目
天津市教委资助项目(2008ZD21) （2008ZD21）
天津市农委资助项目(201004040) （201004040）
