华北农学报2011，Vol.26Issue(3)：204-209,6.
我国长江流域PRRSV ORF3基因的遗传变异分析
Genetic Variations of ORF3 Gene of PRRSV Isolates from Changjiang River Basin in China
摘要
Abstract
The 0RF3 genes of 29 PRRSV,isolated from Changjiang river basin in China from 2006,were amplified , cloned and sequenced with RT-PCR for analyzing the genetic variation of PRRSV, and blast with the representative isolates. The results showed that 0RF3 of all isolates encode the minor structural proteins GP3 ,254 amino acids. All 29 PRRSV isolates belonged to the North American genotype, while 84.6% -100% amino acid identity with each other. It shared 85.0% -97.2% sequence identity with VR-2332,the representative North American strains of PRRSV. And it shared 86.6% -100% sequence identity with the highly pathogenic PRRSV. The phylo-genetic trees indicated that the 29 PRRSV were further divided into two subgenotypes, 28 isolates posses a same branch with the highly pathogenic PRRSV, and HZ24 shares a more closely relationship with RespPRRS vaceine. Meanwhile, there are mutation in the epitopes and the antigenic index of GP3 in all 29 isolates.关键词
猪繁殖与呼吸综合征病毒/ORF3基因/遗传变异/抗原分析Key words
PRRSV/0RF3 gene/Genetic variation/Antigenic analysis分类
农业科技引用本文复制引用
李彬,陈焕春,何孔旺,温立斌,茅爱华,王小敏,张雪寒,倪艳秀,周俊明,肖少波..我国长江流域PRRSV ORF3基因的遗传变异分析 [J].华北农学报,2011,26(3):204-209,6.基金项目
国家自然科学基金项目(31001066) （31001066）
江苏省农业科技自主创新资金(cx(09)619) （cx(09）
国家农业公益性行业专项(200803020) （200803020）
