植物研究2011，Vol.31Issue(4)：397-402,6.
大豆中β-伴大豆球蛋白提纯方法的优化
Optimization of Method for Extraction and Isolation of β-conglycinin from Soybean Seed
摘要
Abstract
β-conglycinin, one of the major storage proteins in soybean seeds, greatly influences the quality of soybean seed proteins. However, increasing the purity of the protein has always been one main problem affecting its characterization and identification. In this study, the purity of β-conglycinin was obviously increased by performing condition optimization of the two key links in the process of extraction and isolation of the protein, based on the method reported by Liu et al. Firstly, SBS at increased concentrations was added to the protein solution before the removing of glycinin ( 11S) , the effect of concentration increase on the purity of p-conglycinin was analyzed , and the optimal concentration was determined as 0. 03 mmol · L-1; Secondly, the mixed glycinin was removed once more by the water precipitation/centrifugation method before precipitating p-conglycinin. The purity of p-conglycinin finally increased to 98% after optimization. The results have important significance for the characterization and identification of β-conglycinin in the genetic research and breeding for soybean proteins.关键词
大豆；β-伴大豆球蛋白；提纯；优化Key words
soybean/ β-conglycinin/ extraction and isolation/ optimization分类
农业科技引用本文复制引用
司晓霞,王志,霍斐,史通麟,付月君,田怀东..大豆中β-伴大豆球蛋白提纯方法的优化[J].植物研究,2011,31(4):397-402,6.基金项目
山西省留学回国人员科技活动择优资助项目(200712)和山西大学人才科研启动基金项目(2005-04-01) （200712）
