中国康复理论与实践2011，Vol.17Issue(9)：840-843,4.
人脑多形性胶质瘤细胞系BT325对5种抗肿瘤药物敏感性的研究
Sensitivity of Human Glioblastoma Multiforme Cell Line BT325 to Antineoplastic Drugs
摘要
Abstract
Objective To examine the sensitivity of human glioblastoma multiforme cell line BT325 to 5 antineoplastic drugs, including cisplatin (DDP), teniposide (VM26), nimustine (ACNU), temozolomide (TMZ) and vincristine (VCR). Methods BT325 cells were incubated in DMEM with 10% or 20% fetal bovine serum (FBS) or without FBS respectively. The cell numbers were counted at 24 h, 48 h, 72 h, 96 h, 120 h, and 144 h, then platting and growth curve were drafted. Cell counting kite-8 was used to detect the influence of 5 drugs with different concentrations on human glioma cell line BT325. Results DDP and VM26 significantly suppressed BT325 cells(>75%) viability in a dose-dependent manner, while VCR inhibited BT325 cells (50%) growth without dose-effect relationship. In contrast, ACNU and TMZ were not effective on the viability of BT325 cells. Conclusion BT325 cells were very sensitive to chemotherapeutic drugs DDP amd VM26.关键词
BT325细胞/抗肿瘤药/药物敏感性/CCK-8法Key words
BT32S cells/ antineoplastic drugs/ drug sensitivity/ cell-countykit-8分类
医药卫生引用本文复制引用
韩明,袁芳,董丽萍,师忠芳,袁辉,杨少华..人脑多形性胶质瘤细胞系BT325对5种抗肿瘤药物敏感性的研究[J].中国康复理论与实践,2011,17(9):840-843,4.基金项目
1.北京市自然科学基金资助项目(7102028) （7102028）
2.北京市卫生系统高层次卫生技术人员培养计划(2009-3-23). （2009-3-23）
