湖南农业大学学报（自然科学版）2011，Vol.37Issue(4)：400-402,3.DOI:10.3724/SP.J.1238.2011.00400
南方水稻黑条矮缩病毒外壳蛋白p1O的原核表达和抗血清制备及应用
Prokaryotic expression of p1O protein of Southern rice black-streaked dwarf Fijivirus and preparation and application of its antiserum
摘要
Abstract
Protein p10 is encoded by the genome segment S10 of Southern rice black-streaked dwarf virus (SRBSDV).Based on the sequence of S10 (EU523360), a pair of S10 specific primers were designed and used to amplify the p10 protein-encoding gene. The expected product was subcloned into the expression vector pET-28a+, which was transformed into Escherichia coli BL21plysS and expressed at high level. Antiserum specifically against plO protein was prepared using the purified protein to immunize rabbit. ELISA tests showed that the antiserum against plO protein could react strongly with fluids from diseased insects, indicating that the antiserum could be used to rapidly diagnose the SRBSDV infection in fields.关键词
南方水稻黑条矮缩病毒/原核表达/抗血清Key words
Southern rice black-streaked dwarf virus (SRBSDV)/ prokaryotic expression/ antiserum分类
农业科技引用本文复制引用
张蔚明,刘燕娟,周倩,廖晓兰..南方水稻黑条矮缩病毒外壳蛋白p1O的原核表达和抗血清制备及应用[J].湖南农业大学学报（自然科学版）,2011,37(4):400-402,3.基金项目
农业部农业行业科技计划项目(201003031-1) （201003031-1）
湖南省科学技术厅项目(2010NK3021) （2010NK3021）
