吉林农业大学学报2011，Vol.33Issue(4)：403-407,5.DOI:CNKI:22-1100/S.20110608.1604.002
正交试验法筛选蛤蟆油粗多糖酶解提取工艺的研究
A Study on Zymohydrolysis Extracting Technology of Polysaccharides from Oviductus Ranae by L9 (34) Orthogonal Design
摘要
Abstract
In order to investigate the optimization of zymohydrolysis1 extracting technology of polysaccharides from Oviductus Ranae by trypsinase, water fraction, trypsinase concentration, zymohydrolysis time and zymohydrolysis pH were taken as factors for Lg(34) orthogonal design trials. The indexes of extracting output and purity of polysaccharides were examined. Results showed that the optimum conditions for the highest output (7.2%) were: the ratio of water to sample was 1 :200, trypsinase concentration was 11% ; zymohydrolysis time was 12 h, and zymohydrolysis pH was 7.5. The optimum conditions of the highest purity (16.9%) were: the ratio of water to sample was 1 :300; trypsinase concentration was 11%; zymohydrolysis time was 4 h, and zymohydrolysis pH was 7.5.关键词
蛤蟆油；多糖；酶解；提取工艺；L9(34)正交试验Key words
Oviductus Ranae/ polysaccharides/ zymohydrolysis/ extracting technology/ I-9(34) orthogonal design分类
医药卫生引用本文复制引用
杨靖,李硕,陈大勇,卫功庆..正交试验法筛选蛤蟆油粗多糖酶解提取工艺的研究[J].吉林农业大学学报,2011,33(4):403-407,5.基金项目
吉林省科技发展计划项目(08SYS-097) （08SYS-097）
