山东医药2011，Vol.51Issue(33)：19-21,3.
RANKL和M-CSF诱导THP-1细胞向破骨细胞样细胞分化的研究
Differentiation of THP-1 cells into osteoclast-like cells induced by RANKL and M-CSF
摘要
Abstract
Objective To establish a method that can induce THP-1 cells to differentiate into osteoclast-like cells efficiently. Methods Firstly, THP-1 cells was induced to differentiate into adherent cells by TPA, then differentiated into osteoclast-like cells induced by RANKL and M-CSF. Lastly. Using tartrate-resistant acid phosphatase (TRAP) method to determine whether the multinuclear-cells were osteoclast-like cell or not. Results THP-1 cell could differentiate into osteoclast-like cells under the stimulation of RANKL and M-CSF. Conclusions The expression level of RANK in THP-1 cells is high enough for it to response to RANKL. THP-1 cells can differentiate into osteoclast-like cells induced by RANKL and M-CSF.关键词
THP-1/破骨细胞生成因子/巨噬细胞集落刺激因子/破骨细胞样细胞Key words
THP-1/ RANK/ M-CSF/ Osteoclast-like cell分类
医药卫生引用本文复制引用
刘天云..RANKL和M-CSF诱导THP-1细胞向破骨细胞样细胞分化的研究[J].山东医药,2011,51(33):19-21,3.基金项目
国家自然科学基金资助项目(30600716). （30600716）
