南京医科大学学报（自然科学版）2011，Vol.31Issue(10)：1399-1402,4.
IRE1α通过UPR影响非洲爪蟾胚胎发育
The role of IRE1α on embryo development of xenopus lavies
摘要
Abstract
Objective: To explore whether IREloi can mediate unfolded protein response (UPR) during embryo development of xenopus laevis and rescue the developmental defects induced with tunicamycin. Methods; IRE la mRNA or specific antisense oligonucleotids morpholino were microinjected to the 4 blastomeres of 4-cell stage embyos to overexpress or knockdown gene expression; the splicing of XBP1 was tested with RT-PCR to reflect the UPR;Tunicamycin was used to treat the embryos to induce the endo-plasmic reticulum stress (ERS). Results; The splicing of XBP1 was increased when IRE la was overexpressed while the splicing was decreased when IRE la was knockdwon. Embryos developmental defects and the increase of XBP1 splicing were induced with tunicamycin while knockdown of XBP-1 partially rescued the defects and knockdown of IRE la rescued the defects significantly and the increased XBP1 splicing. Conclusion; IRE la can mediate UPR during xenopus development. Knockdown of IRE la rescued the developmental defects induced with TM and partially by the IREla/XBPl pathway.关键词
IRE1α/非洲爪蟾/胚胎发育Key words
IRE 1 a/ xenopus laevis/ embryo development分类
生物科学引用本文复制引用
李昕昕,冯娇娇,侯道荣,郭静,王学军,王宁,袁栎..IRE1α通过UPR影响非洲爪蟾胚胎发育[J].南京医科大学学报（自然科学版）,2011,31(10):1399-1402,4.基金项目
国家自然科学基金资助(30971680) （30971680）
