中国兽医科学2011，Vol.41Issue(6)：610-613,4.
马流感病毒NP单克隆抗体的制备及特性鉴定
Preparation and identification of monoclonal antibodies against NP of equine influenza virus
摘要
Abstract
Four week-old BALB/c mice were intraperitoneally immunized with the prokaryoticly-ex-pressed NP four times. Three hybridoma cell lines named 2G11,3E10 and 4A1 were obtained by fusing SP2/0 with spleen cells. The first monoclonal antibody(McAb) 2G11 was identified to be IgGl,3El to be IgG2a and 4A1 to be IgM,all of them with k light chain. The antibody titres of their hybridoma supernatant and ascites were 1:640,1:640,1:320 and 1:409 600,1:204 800,0,respectively. Western-blot analysis showed that the three McAbs could react with the recombinant NP specifically. IFA showed that they could combine with the natural EIV well. The obtained 3 McAbs had no cross reactions with equine arteritis virus, equine herpes virus-1, equine herpes virus-4 and Japanese encephalitis virus. The affinity test showed that 2G11 and 3E10 were 4. 39×106 M-1 and 2. 20×106M-1 in affinity constant, respectively.关键词
马流感病毒/核蛋白/单克隆抗体/特性鉴定Key words
equine influenza virus /nucleoprotein/ monoclonal antibody characterization identification分类
农业科技引用本文复制引用
姬媛媛,郭巍,王征,赵立平,李红梅,相文华..马流感病毒NP单克隆抗体的制备及特性鉴定[J].中国兽医科学,2011,41(6):610-613,4.基金项目
黑龙江省自然科学基金重点项目(ZD200812) （ZD200812）
