土壤学报2011，Vol.48Issue(4)：804-812,9.
石油污染土壤鞘氨醇单胞菌遗传多样性16SrDNA-PCR-DGGE分析
ANALYSIS OF SPHINGOMONAS GENETIC DIVERSITY IN PETROLEUM-CONTAMINATED SOILS BY USING PCR-DGGE TECHNIQUE
摘要
Abstract
Members of Sphingomonas genus played a key role in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). However, little was available in the literature about diversity and population structure of genus Sphingomonas in petroleum-contaminated soils, and relationships between Sphingomonas and PAHs based on direct detection Sphingomonas strains with Sphingomonas genus-specific primers. In this paper, total soil DNA from the soil in Shenfu irrigation zone were amplified with newly designed Sphingomonas genus-specific primer set 732f-982r and then these amplified DNA fragments were separated by DCCE for analysis of genetic diversity of Sphingomonas in the soil of the irrigation zone. Results indicate that the newly developed primer set 732f-982r could be used for specific detection of strains belonging to the genus Sphingomonas. When PAHs contamination of the soil is at a low level, I. E. Less than 5 mg kg-1, the diversity of Sphingomonas increased with rising PAHs concentration; but when the initial concentration of PAHs in lab incubation experiment is relatively high, the diversity of Sphingomonas decreased. Different concentrations of total petroleum hydrocarbons (TPH) lead to difference in Sphingomonas population, and soils the same in TPH contamination level are similar in Sphingomonas population.关键词
石油污染土壤/鞘氨醇单胞菌/遗传多样性/变性梯度凝胶电泳/总石油烃/多环芳烃Key words
Petroleum-contaminated soils/ Sphingomonas/ Genetic diversity/ Denaturing gradient gel electrophore-sis (DGGE) / Total petroleum hydrocarbons (TPH) / Polycyclic aromatic hydrocarbons (PAHs)分类
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周丽沙,李慧,张颖,王亚菲,徐慧..石油污染土壤鞘氨醇单胞菌遗传多样性16SrDNA-PCR-DGGE分析[J].土壤学报,2011,48(4):804-812,9.基金项目
国家自然科学基金青年科学基金项目(30800157)、国家自然科学基金项目(30670391)和中国科学院陆地生态过程重点实验室基金项目资助 （30800157）
