中国药房2011，Vol.22Issue(26)：2441-2442,2.
HPLC法测定人血清中丙戊酸钠的浓度
Determination of Sodium Valproate in Human Serum by HPLC
周莉华 1涂琼 2梅步云1
作者信息
- 1. 江西省人民医院,南昌市330006
- 2. 江西省儿童医院,南昌市330006
- 折叠
摘要
Abstract
OBJECTIVE: To establish the method for the concentration determination of sodium valproate in human serum. METHODS: The samples were derivatized with 2-Bromo-4' -nitroacetophenone, and HPLC method was adopted. The chromato-graphic column was Shimpack C18(150 mm×4.6 mm, 5 urn) column with mobile phase consisted of methanol-water (80 :20) at flow rate of 0.9 mL·min-1. The colum temperature was 30 °C and the detection wavelength was set at 265 nm. The cyclohexanecar-boxylic acid was used as internal standard. RESULTS:The linear range of sodium valproate was 10-150 mg·L-1(r=0.999 7,w= 6). The minimum detectable concentration was 1 mg·L-.1 The average recovery of relative and extraction was 99.45% -103.24% and 98.12%~100.37%. The RSD of intra-day and inter-day were less than 8%(n=5). CONCLUSION: The method is rapid, simple and accurate, which can be used for serum concentration monitoring of sodium valproate.关键词
高效液相色谱法/丙戊酸钠/血药浓度/药动学Key words
HPLC/ Sodium valproate/ Serum concentration/ Pharmacokinetics分类
医药卫生引用本文复制引用
周莉华,涂琼,梅步云..HPLC法测定人血清中丙戊酸钠的浓度[J].中国药房,2011,22(26):2441-2442,2.
