中国临床药理学与治疗学2011，Vol.16Issue(9)：965-970,6.
A771726对体外培养胶原性关节炎大鼠腹腔巨噬细胞产生一氧化氮的影响
In vitro effect of A771726 on the secretion of nitric oxide of peritoneal macrophages from collagen-induced arthritis rats
王婷玉 1李俊 2金芝贵 2吴飞华 1王小华 1周倩2
作者信息
- 1. 上海交通大学医学院附属第九人民医院药剂科,上海200011
- 2. 安徽医科大学药学院,合肥230032,安徽
- 折叠
摘要
Abstract
To study the effect and mechanism of A771726, the active metabolite of leflunomide, on the production of nitric oxide (NO) of peritoneal macrophages (PMΦ) from collagen-induced arthritis (CIA) rats. METHODS- Collagen-induced arthritis (CIA) model was induced in rats and the PMO were collected. A771726 were used in vitro. The dose-dependent and time-dependent effects of A771726 on the secretion of NO of PMO from CIA rats were checked by nitric acid reduction synthase method; MTT reagent was used to detect the effect of A771726 on the cell viability of PMO from CIA rats:RT-PCR was used to detect the expression of inducible NO synthase (iNOS) mRNA. Western blot was used to detect the expression of iNOS protein. RESULTS: A771726could inhibit the secretion of NO of PMΦ from CIA rats in a dose-dependent and time-dependent manner. The cytotoxicity of A771726 was not detected during the time course and dose range. A771726 (0.1, 1, 10 mol/L) significantly inhibited the expression of iNOS mRNA and iNOS protein in PMO from CIA rats. CONCLUSION: A771726 (0.1, 1, 10 mol/L) can inhibit the transcription of iNOS mRNA in PMΦ from CIA rats, and reduce the translation of iNOS protein, which leads to suppressing the secretion of NO. And the suppression is not related to the cytotoxicity of A771726.关键词
A771726/关节炎/巨噬细胞/诱导型一氧化氮合酶Key words
A771726/ Arthritis/ Macrophages/ iNOS分类
医药卫生引用本文复制引用
王婷玉,李俊,金芝贵,吴飞华,王小华,周倩..A771726对体外培养胶原性关节炎大鼠腹腔巨噬细胞产生一氧化氮的影响[J].中国临床药理学与治疗学,2011,16(9):965-970,6.
