哈尔滨商业大学学报（自然科学版）2011，Vol.27Issue(3)：259-262,275,5.
银翘散体外鸡胚成纤维细胞最大安全质量浓度
Safety concentration of lonicerae and forsythiae powder in vitro determination on CEF
韩雪 1李文兰 2丁振铎1
作者信息
- 1. 国家教育部抗肿瘤天然药物工程研究中心，哈尔滨150076
- 2. 哈尔滨商业大学生命科学与环境科学研究中心，哈尔滨150076
- 折叠
摘要
Abstract
Using in vitro cell culture method and four methyl azo thiazole salt MTT eolorimet ric analysis to research the maximum security concentration of Yinqiao scattered and its main ingredients in vitro of CEF. Using optical inverted microscope to observe the shape of the cells by naked eye, and analysis whether the drug can restrain proliferation phenomenon of cell infection caused by the virus. The MTT method uses the principle that reactive dyes only dying living cells and the viral infection destruction of cells will not be dying, determine light density absorption OD values after a period of time, then calculate the damage percentage of cell. The cytotoxicity of Yinqiao scattered was very low, almost does not produce toxic effects to CEF. The maximum security concentration was 62. 5 mg/mL, but the maximum security concentration was 15. 625 mg/mL by MTT method. At a high concentration ( Respectively than 15. 625, 15. 625, 125 mg/mL) ,it showed that toxicity to CEF cells, at a low concentration (Respectively was 7. 81, 3. 906 mg/mL) ,it had effect of growth promoting and prolong survival time to CEF cells. MTT method was more objective than morphology observation and could be used, it also had higher sensitivity.关键词
银翘散；鸡胚成纤维细胞；最大安全质量浓度；中药；体外Key words
Yinqiao powder/ chicken embryo finbroblast/ maximum safety concentration/ herbs/ in vitro分类
医药卫生引用本文复制引用
韩雪,李文兰,丁振铎..银翘散体外鸡胚成纤维细胞最大安全质量浓度[J].哈尔滨商业大学学报（自然科学版）,2011,27(3):259-262,275,5.
