哈尔滨商业大学学报（自然科学版）2011，Vol.27Issue(3)：279-281,3.
光谱法研究杨梅酮与黄嘌呤氧化酶相互作用
Study on interaction between myricetin and XO via spectroscopy
梁启超 1沈广志 1魏涛 2武冬梅3
作者信息
- 1. 牡丹江医学院药学院，黑龙江牡丹江157011
- 2. 牡丹江医学院基础学院，黑龙江牡丹江157011
- 3. 佳木斯大学药学院，黑龙江佳木斯154007
- 折叠
摘要
Abstract
The interaction between myricetin and xanthine oxidase ( XO) was studied under the physiological conditions ( pH 7. 4 ) by UV - Vis absorption spectrometry. With the in creasing time of XO added to myricetin, obvisious hypochromic effect of I Band (376 nm) and II Band (266nm) were observed in the UV - Visible absorption spectra of myricetin. At the same time, a new absorption peak gradually increased at 331 nm, the absorbance in creased from 0. 649 to 1.053 (0 to 29 min). The interaction between myricetin and xanthine oxidase was enzymatic reaction, myricetin was the "suicide" substrate of xanthine oxidase, which indicates that the reaction rate is limited by the amount of xanthine oxidase.关键词
杨梅酮；黄嘌呤氧化酶；紫外-可见光谱；相互作用Key words
myricetin/ XO/ UV - Vis absorption spectroscopy/ interaction分类
医药卫生引用本文复制引用
梁启超,沈广志,魏涛,武冬梅..光谱法研究杨梅酮与黄嘌呤氧化酶相互作用[J].哈尔滨商业大学学报（自然科学版）,2011,27(3):279-281,3.
