中医学报Issue(10)：1217-1218,2.
薄层扫描法测定天然牛黄与人工牛黄胆酸含量比较
Comparison on the Contents of Cholic Acid in Natural Calculus Bovis and Calculus Bovis Artifactus Determined by Thin Layer Chromatography Scanning
王志强 1刘海津2
作者信息
- 1. 新乡市第二人民医院，河南新乡453000
- 2. 河南师范大学化学与环境科学学院，河南新乡453000
- 折叠
摘要
Abstract
Objective:To determine and compare the total contents of cholic acid in natural calculus bovis and calculus bovis artifactus in 6 product units. Methods: The silica G thin layer plate was used with isooctane - butyl acetate - glacial acetic acid - formic acid (8 : 4 : 2 : 1) as developing solvent, λs =380 nm,λR =650 nm,in the reflection of jagged scanning. Results:The content of cholic acid in calculus bovis artifactus was the same as that in natural calculus bovis. The content of free bile acid in calculus bovis artifactus was more than that in natural calculus bovis. Therefore, calculus bovis artifactus could be the substitute for natural calculus bovis within limits. Conclusion: Thin layer chromatography ( TLC ) scanning is a reliable method to determine the contents of cholic acid in natural calculus bovis and calculus bovis artifactus.关键词
薄层扫描法；天然牛黄；人工牛黄；胆酸Key words
TLC scanning/natural calculus bovis/calculus bovis artifactus/cholic acid分类
医药卫生引用本文复制引用
王志强,刘海津..薄层扫描法测定天然牛黄与人工牛黄胆酸含量比较[J].中医学报,2011,(10):1217-1218,2.
