中国临床药理学与治疗学2011，Vol.16Issue(9)：975-980,6.
高迁移率族蛋白A2在肿瘤发生和血管形成中的双重作用
HMGA2 play important roles both in tumorigenesis and angiogenesis
摘要
Abstract
AIM: To analyze the roles of high mobility group A2 protein (HMGA2) in tumorigenesis and angiogenesis. METHODS: 5 siRNAs targeting HMGA2 gene ( HMGA2 siRNAl-5) were designed, synthesized, and transfected into cells using Lipofactamine 2000. MTT assay, transwell assay and tube formation assay were applied to evaluate the effects of these siRNAs on cancer cells (SPC-A1 and Hela) and endothelial cells (ECV-304 and HUVEC). RESULTS: HMGA2 siRNAl, 3 and 5 inhibited the growth of all the cell lines that were tested. Among these siRNAs, HMGA2 siRNA5 show the best inhibition effect. The relative growth ratios were63.2%±6.5% (P<0.01), 86.8%±3.5% (P <0. 01), 58. 5% ±6. 3% (P<0. 01), and 60.3^0 + 7.3% (P<0. 01) for Hela, SPC-A1, EVC-304 and HUEVC cells, respectively. Furthermore, interfering the expression of HMGA2 affected the growth, migration, and tube formation of endothelial cells (P<0.01). CONCLUSION; HMGA2 protein plays important roles both in tumorigenesis and angiogenesis.关键词
高迁移率族蛋白A2/血管形成/肿瘤发生/RNA干扰/基因治疗Key words
HMGA2f Angiogenesis/ Tumorigenesis/ RNAi/ Gene therapy分类
医药卫生引用本文复制引用
吕颖慧,龚玉华,刁勇,许瑞安,王启钊..高迁移率族蛋白A2在肿瘤发生和血管形成中的双重作用[J].中国临床药理学与治疗学,2011,16(9):975-980,6.基金项目
国家自然科学基金(30900822) （30900822）
福建省高校杰出青年人才培育计划(王启钊) （王启钊）
华侨大学校基金(09BS517、基地专项)资助项目 （09BS517、基地专项）
