中国临床药理学与治疗学2011，Vol.16Issue(9)：981-985,5.
哌唑嗪和去氧肾上腺素对大鼠肝星状细胞增殖和凋亡的作用
Effects of prazosin and phenylephrine on the proliferation and apoptosis of rat hepatic stellate cells
陈猛 1马勇2
作者信息
- 1. 嘉兴市第一医院药剂科,嘉兴314000,浙江
- 2. 中国人民解放军第123中心医院,蚌埠233015,安徽
- 折叠
摘要
Abstract
AIM: To investigate the effects of agonist and antagonist of radenoreceptor (α1-AR) on the proliferation and apoptosis of rat hepatic stellate cells (HSC-T6). METHODS; After the drug was administered to the cultured HSC-T6; MTT assay was used to α1 -evaluate the proliferation of HSC-T6; ELISA assay was used to evaluate the produce of cAMP in HSCT6; flow cytometer (FCM) was used to test the apoptosis of HSC-T6; the expression of nuclear factor-κBp65 (NF-κBp65) protein was detected by immunocytochemical technique. RESULTS:In the in vitro experiments, phenylephrine (10-5, 10-71 10-9 mol/L) significantly decreased the produce of cAMP in HSC-T6, promoted HSCT6 proliferation, enhanced the expression ofNF-κBp65 protein and inhibited the apoptosis of HSC-T6, on the contrary to the antagonist prazosin ( 10-5, 10-7, 10-9 mol/L) of α1-AR. CONCLUSION; Phenylephrine can promote proliferation and inhibit apoptosis of the cultured HSC-T6, while prazosin has opposite effect. Its mechanism might be associated with the α1-adenoreceptor by meditating the produce of cAMP and the expression of nuclear factor kappa Bp65 in HSC-T6.关键词
肝星状细胞/增殖/凋亡Key words
Hepatic stellate cell/ Proliferation/ Apoptosis分类
医药卫生引用本文复制引用
陈猛,马勇..哌唑嗪和去氧肾上腺素对大鼠肝星状细胞增殖和凋亡的作用[J].中国临床药理学与治疗学,2011,16(9):981-985,5.
