中国组织工程研究与临床康复2011，Vol.15Issue(41)：7611-7614,4.DOI:10.3969/j.issn.1673-8225.2011.41.003
胰岛素样生长因子1与转化生长因子β2促进组织工程软骨构建的体外实验
Insulin-like growth factor 1 and transformation growth factor beta 2 promote engineered cartilage formation in vitro
摘要
Abstract
BACKGROUND: Cartilage tissue has poor regenerative capacity. Cartilage tissue engineering can repair damaged cartilage tissue using fewer cells, scaffold materials and cytokines. OBJECTIVE: To investigate the effects of insulin-like growth factor (IGF-1) combined with transformation growth factor beta 2 (TGF-2) on engineered cartilage formation. METHODS: Human chondrocytes were isolated by enzyme digestion method and then were inoculated onto calcium alginate bead scaffold for culture in three-dimensional circumstance after addition of 200 μg/L IGF-1 and (or) 1 μg/L TGF-2. At 3, 5, 7, 9, 11, and 13 days of culture, chondrocyte proliferation was tested by cell counting. After 2 weeks of culture, the fresh tissues were assessed by morphological observation, alcian blue-periodic acid sthiff staining, and anti typeⅡcollagen immunohistochemicel staining. RESULTS AND CONCLUSION: IGF-1 and TGF-2 could stimulate chondrocytes to proliferate and excrete chondral matrix. IGF-1 mainly promoted the proliferation of chondrocytes, while TGF-2 mainly promoted the formation of cartilage-related matrix. IGF-1 combined with TGF-2 has synergistic effects on promoting engineered cartilage formation.关键词
胰岛素样生长因子1/转化生长因子β2/软骨/藻酸钙/软骨组织工程分类
基础医学引用本文复制引用
陈宁杰,张伟,梁晓琴,杨彪炳..胰岛素样生长因子1与转化生长因子β2促进组织工程软骨构建的体外实验 [J].中国组织工程研究与临床康复,2011,15(41):7611-7614,4.基金项目
山东省高等学校科技计划项目(J09LF20),课题名称:藻酸钙凝珠载体软骨细胞立体培养及调控因素的研究. （J09LF20）
