农药学学报2011，Vol.13Issue(4)：394-401,8.DOI:10.3969/j.issn.1008-7303.2011.04.11
霉毒素B1液相芯片定量检测方法的探索
Quantitative analysis of Aflatoxin B1 using the microsphere array technology
摘要
Abstract
The quantitative detection of Aflatoxin Bl (AFB1) was investigated on the microsphere array platform and indirect competition by using the Anti-Aflatoxin Bl. Through optimizing the concentration and saturated concentration of AFB1-BSA and Anti-Aflatoxin Bl as well as incubation time of antigen and antibody, the method of quantitative detection of AFB1 was established. As referring to the detection sensitivity standard IC50, results showed that the detection sensitivity was 1.33 ng/mL. Meanwhile,as referring to the limit of detection standard IC]0 ,the detection limition was 0.15 ng/mL. The linear equation was v =0. 000 6x + 0. 000 8. By applying this original research to test the imitating contaminated skim and full milk,the recovery rate of the two kinds of milk was all greater than 75% under the AFB1 added level of 2. 0 - 16. 0 ng/mL, and the relative standard deviation was between 2. 80% -4. 69% (n =7). Overall, the quantitative analysis of AFB1 using the microsphere arraytechnology was sensitive,stable,rapid and simple. The method was applicable to mass sample testing.关键词
黄曲霉毒素B1/液相芯片技术/AFB1多克隆抗体/定量分析Key words
Aflatoxin Bl /microsphere array technology /Anti-Aflatoxin Bl quantitative analysis分类
生物科学引用本文复制引用
宋慧君,马惠蕊,裴轶君,刘淑艳,曹远银..霉毒素B1液相芯片定量检测方法的探索[J].农药学学报,2011,13(4):394-401,8.基金项目
中国质检总局资助项目(2009IK152) （2009IK152）
