河南农业大学学报2011，Vol.45Issue(3)：297-301,5.
河南省栽培平菇品种的种质资源评价
Evaluation of germplasm resources with cultivated Pleurotus spp.strains in Henan Province
摘要
Abstract
Using the methods of antagonism reaction, esterase isozyme electrophoresis analysis, ISSR and ITS sequence analysis, studies on the taxonomic identification and genetic diversity were conducted with the 61 cultivated Pleurotus spp. Strains in Henan province. They were divided into 13 vegetative compatibility groups according to antagonism reaction. The esterase isozyme electrophoresis analysis showed that the isozymograms of the non-antagonism strains showed no difference. However, the esterase isozymograms of 13 strains chosen from 13 vegetative compatibility groups were different. 106 loci were amplified through ISSR augmented, and the percentage of polymorphism loci (P) was 95.4%. The sequence amplified from the 13 chosen strains with ITS primer blasted and showed that one of the 13 strains is Pleurotus ostreatus, 4 strains are Pleurotus florida, 1 strain is Pleurotus pulmonarius and 7 strains are Pleurotus conucopinus. The results of cultivation indicated that the morphological characteristics of these 13 strains were significantly different and they can be separated into different species of Pleurotus, but no obvious difference was observed in the strains in the same vegetative compatibility groups. The results showed that 61 cultivated Pleurotus spp. Strains in Henan Province belong to 13 vegetative compatibility groups in four Pleurotus types, besides, with a rich genetic diversity.关键词
平菇/遗传多样性/拮抗/酯酶同工酶Key words
oyster mushroom/ genetic diversity/ antagonism/ esterase isozyme分类
农业科技引用本文复制引用
申进文,赵旭,李燕,关园园,王震,戚元成,张金霞..河南省栽培平菇品种的种质资源评价[J].河南农业大学学报,2011,45(3):297-301,5.基金项目
现代农业产业技术体系建设专项资金资助(无编号) （无编号）
