河南农业大学学报2011，Vol.45Issue(3)：302-306,5.
河南地区猪戊型肝炎病毒的检测及基因序列分析
Detection and sequence analysis of the swine HEV in Henan
摘要
Abstract
133 swine serum samples collected from Zhengzhou, Luoyang, Xinxiang, Jiaozuo and Zhoukou were tested by ELISA for the presence of IgG antibodies against HEV. 200 pig fecal samples from Zhengzhou, Xinzheng, Zhongmou, Jiyuan and Puyang were tested for the presence of HEV RNA by reverse transcription nested polymerase chain reaction ( RT-PCR). Results showed 79.70% of the serums were positive for antibody against HEV,and 7.5% of the fecal samples were positive for HEV RNA. Nucleotide sequence analysis showed that three HEV strains detected in Henan belong to genotype Ⅳ. To get the characteristics of Henan HEV, we cloned 826 bp of 3' sequence from JY40 genome, and phylogeny analysis showed it had a high identity with Hubei strain.关键词
猪/戊型肝炎病毒/检测/基因序列分析Key words
swine/ HEV/ detection/ sequence analysis分类
农业科技引用本文复制引用
张小霞,许锋,梁振普,乔冠华..河南地区猪戊型肝炎病毒的检测及基因序列分析[J].河南农业大学学报,2011,45(3):302-306,5.基金项目
河南省重大公益性科研项目(81100912300) （81100912300）
