南京医科大学学报（自然科学版）2011，Vol.31Issue(10)：1521-1526,6.
高效液相色谱编程荧光法测定室尘中痕量多环芳烃
Determination of polycyclic aromatic hydrocarbons in indoor dust by high-performance liquid chromatography with fluorescence detection and wavelength programming
摘要
Abstract
Objective; To find out the optimal separating conditions and wavelength program for measurement of 16 polycyclic aromatic hydrocarbons in house dust by high-performance liquid chromatography (HPLC) with fluorescence detector. Methods: Vacuum cleaner was used for gathering the house dust,and acetone was added and extraction was performed in a ultrasonic bath. Trie supernatant was concentrated and then added methyl cyanide to 0.1 ml. With methyl cyanide and water as mobile phase,PAHs were efficiently separated by gradient elution and detected by fluorescence detector and wavelength programming. Results; Except for aee-naphthylene, 15 PAHs had highly efficient separation within 28 min and showed good linearity with 0.5-50 ng/ml range. The relative standard deviations were 1.35%-5.89%,and the recoveries were 62.45%-110.36%. Conclusion; HPLC with fluorescence detection and wavelength programming could be used for detection of PAHs in house dust because of its quickness,simplicity,sensitivity and reproducibility.关键词
高效液相色谱法/室尘/多环芳烃Key words
high-performance liquid chromatography/house dust/polycyclic aromatic hydrocarbons分类
医药卫生引用本文复制引用
陆晓梅,王炳玲,张晓玲,张绮..高效液相色谱编程荧光法测定室尘中痕量多环芳烃[J].南京医科大学学报（自然科学版）,2011,31(10):1521-1526,6.基金项目
基金项目国家自然科学基金面上项目(81072268) （81072268）
江苏省自然科学基金面上项目(BK2010535) （BK2010535）
