南方医科大学学报2011，Vol.31Issue(10)：1701-1704,4.DOI:44-1627/R.20111003.0951.001
Apollon siRNA提高肝癌细胞化疗敏感性的实验研究
Small interfering RNA targeting Apollon enhances the chemosensitivity of hepatocellular carcinoma cells in vitro
摘要
Abstract
Objective To investigate the effect of small interfering RNA (siRNA) targeting Apollon in enhancing the chemosensitivity of hepatocellular carcinoma (HCC) cells in vitro. Methods HCC cells transfected with the siRNA targeting Apollon were tested for Apollon protein expression using Western blotting. Mil assay and ELISA were used to evaluate the proliferation and apoptosis of HCC cells trasnfected with siRNA after exposure to 5-FU or adriamycin. Results Apollon siRNA obviously down-regulated Apollon protein expression in HCC cells. The siRNA-mediated suppression of Apollon expression resulted in a marked inhibition of cell growth and increased apoptotic rate of HCC cells, and enhanced both the growth-inhibiting and apoptosis-inducing effects of the chemotherapeutic drugs. Conclusion Apollon siRNA can enhance the chemosensitivity of HCC cells to the chemotherapeutic drugs. Apollon can be a potentially important and feasible therapeutic target for HCC.关键词
Apollon/小干扰RNA/肝癌/化疗敏感性Key words
Apollon/ small interfering RNA/ hepatocellular carcinoma/ chemosensitivity分类
医药卫生引用本文复制引用
陈建发,李宇华,陈引香,谢奎龙,傅明,李立..Apollon siRNA提高肝癌细胞化疗敏感性的实验研究[J].南方医科大学学报,2011,31(10):1701-1704,4.基金项目
国家973计划前期研究专项课题(2010CB535002) （2010CB535002）
