中国组织工程研究与临床康复2011，Vol.15Issue(45)：8371-8375,5.DOI:10.3969/j.issn.1673-8225.2011.45.003
骨髓间充质干细胞下调类风湿性关节炎小鼠脾脏单个核细胞TLR8及TLR9的表达
Effect of bone marrow mesenchymal stem cells on down-regulation of Toll-like receptor 8 and Toll-like receptor 9 in spleen mononuclear cells in collagen-induced arthritis mice
摘要
Abstract
BACKGROUND: Mesenchymal stem cells (MSCs) can attenuate the symptoms of collagen-induced arthritis (CIA) mice but themechanism of which is not clear.OBJECTIVE: To explore the effect of MSCs on the expression of Toll-like receptor 8 (TLR8), Toll-like receptor 9 (TLR9) and soon in spleen mononuclear cells in CIA mice.METHODS: DBA/1 J mice were randomly divided into sham-operation group, positive control group and test group. Chicken IIcollagen was used to establish the model of CIA in mice. Rat MSCs were administered into the test group through intravenousinjection.RESULTS AND CONCLUSION: The joint diameter of the test mice decreased obviously compared with that of positive controlgroup. Severe inflammation was detected in the positive group mice by histological analysis, which was not seen in the test groupand negative group. The expression levels of TLR8, TLR9 and interleukin-1B in spleen mononuclear cells from the test groupwere decreased compared with those from the positive group which were higher than that from the negative group (P < 0.01, P <0.05). Statistical analysis showed that there was no correlation between the expression of TLR8 and TLR9 in the positive controlgroup and test group (P > 0.05). The expression of TLR8 and TLR9 in spleen mononuclear cells from CIA mice were decreasedby MSCs, and there was no correlation between the expression of TLR8 and TLR9.关键词
类风湿性关节炎/骨髓间充质干细胞/TLR8/TLR9/小鼠分类
基础医学引用本文复制引用
毛飞,钱晖,朱伟,严永敏,高硕,许文荣..骨髓间充质干细胞下调类风湿性关节炎小鼠脾脏单个核细胞TLR8及TLR9的表达[J].中国组织工程研究与临床康复,2011,15(45):8371-8375,5.基金项目
国家自然科学青年基金项目(81000181),课题名称:小鼠骨髓MSC调控单核/巨噬细胞修复肝损伤机制的研究.江苏省高校自然科学研究项目(10KJB310002) ,课题名称:小鼠骨髓MSC调节单核/巨噬细胞修复肝损伤的机制研究.江苏大学高级专业人才科研启动基金项目(10JDG094),课题名称:LacZ转因小鼠骨髓MSC调节单核/巨噬细胞修复肝操作的机制研究.江苏大学拔尖人才和科技创新团队项目(2008-018-02). （81000181）
