河南农业大学学报2011，Vol.45Issue(3)：353-357,5.
拟南芥转录因子AGL47的原核表达与包涵体复性研究
Prokaryotic expression and inclusion body renaturation of the plant transcriptional factor Arabidopsis thaliana AGL47
摘要
Abstract
AGL47 is a transcription factor coded by the At5g55690 gene on the fifth chromosome of Arabidopsis thaliana, belonging to MADS-box protein family. At5g55690 gene was reported to be upregulated by limited inorganic phosphate stress and may play an important role in plant phosphorus metabolism. To identify its function, At5g55690 gene' s full-length CDS was cloned to construct recombinant plasmid pET-28a-At5g55690. pET-28a-At5g55690 was then transformed into E. Coli BL21 ( DE3) cells to perform prokaryotic expression. AGL47 protein was obtained by induction of 1 mmol· L-1 IPTG but found to be in form of inclusion bodies. AGL47 soluble protein was isolated from purified inclusion bodies after denaturation with urea and renaturarion with dialysis.关键词
拟南芥/AGL47/转录因子/原核表达/包涵体复性Key words
Arabidopsis thaliana/ AGL47/ transcription factor/ prokaryotic expression/ inclusion body renaturation分类
农业科技引用本文复制引用
乔帅,王明凤,杨文博,许君,郑文明..拟南芥转录因子AGL47的原核表达与包涵体复性研究[J].河南农业大学学报,2011,45(3):353-357,5.基金项目
河南省杰出青年基金项目(084100410025) （084100410025）
河南省农业科技攻关重点项目(112102110112). （112102110112）
