中国临床药理学杂志2011，Vol.27Issue(10)：785-788,4.
中缝背核参与大鼠吗啡依赖和戒断的形成机制研究
Study on the mechanism of dorsal raphe nucleus to participate in the development of morphine dependence and withdrawal in rat
秦承伟 1张勇 1赵彦明 1高桃 1张如意 1张励才2
作者信息
- 1. 滨州医学院附属医院麻醉科,山东滨州256603
- 2. 徐州医学院麻醉学院,江苏徐州221002
- 折叠
摘要
Abstract
Objective To observe the effect of the dorsal raphe nucleus ( DRN) of rat brain parenchyma in the development of morphine dependence and withdrawal by nitric oxide (NO) mediation. Methods Male adult Sprague - Dawley rats were divided randomly into S groups: withdrawal (morphine + naloxone) group, dependence ( morphine + saline) group, saline (saline) group, naloxone (saline + naloxone) group and L - NAME ( morphine + naloxone + L - NAME ) group. All animals were killed and the relative tissue of rats' brain was removed after the ethology surveying and the scoring of withdrawal symptoms. Frozen serial coronal sections were cut Then neuron nitric oxide synthase (nNOS) was marked by immunohistochemistry. The number of the nNOS positive neurons on the same segmental brain sections were counted. Results The withdrawal symptoms of the withdrawal group were significant. Scores of all signs were significantly higher than the dependence group and control groups (P <0. 01). The withdrawal signs of L - NAME group attenuated obviously compared to that of withdrawal group (P < 0. 05). There was no significant difference in the number of nNOS labeled neurons of saline and naloxone group. The number of nNOS labeled neurons of dependence and withdrawal group increased significantly compared to those of the above groups ( P < 0. 05 ). The number of nNOS labeled neurons of L - NAME( L - nitro - arginine - methyl - ester) group was de-eased significantly compared to that of withdrawal group ( P < 0. 05 ). Conclusion DRN of rat brain parenchyma might partly be participated in the development of morphine dependence and naloxone precipitated withdrawal by NO signal passageway.关键词
中缝背核/吗啡/戒断/一氧化氮合酶Key words
dorsal raphe nucleus/ morphine/ withdrawal/ nitric oxide synthase分类
医药卫生引用本文复制引用
秦承伟,张勇,赵彦明,高桃,张如意,张励才..中缝背核参与大鼠吗啡依赖和戒断的形成机制研究[J].中国临床药理学杂志,2011,27(10):785-788,4.
