中国临床药理学杂志2011，Vol.27Issue(10)：789-792,4.
液相色谱-质谱联用法同时测定生脉注射液中人参皂苷Rg1、Re、Rb1和五味子醇甲在健康人血浆的浓度
Determination the concentration of ginsenoside Rg1, Re, Rb1 and schisantherin A of shengmai injection in healthy human plasma by LC-MS/MS
摘要
Abstract
Objective To develop a LC - MS/MS method for the determination the concentration of ginsenoside Rg1, Re, Rb1 and schisanther-in A of shengmai injection in healthy human plasma. Methods Plasma samples were pretreated by acetonitrile, ginsenoside Rg1, Re, Rb1 and schisantherin A was detected by the positive electrospray ionization method under multiple reaction monitoring mode. Results The calibration curve of ginsenoside Rg,, Re, Rb1 and schisantherin A in plasma was linear over the range of 0. 64 -509.00, 0. 62 -501.00, 1. 18 -4725.00 and 0. 10 - 153.00 μg · L-1 . The limit of quantitation was 0. 64,0. 62, 1. 18 and 0. 10 μg · L-1 . The relative were 78.55% - 91.65% , 78. 57% - 104. 98% ,79. 62% -93. 17% and 82. 50% - 104. 80% , respectively. The within - day and between - day RSDs were less than 15%. Conclusion This method is accurate, sensitive, and specific, it is suitable for the measurement of plasma ginsenoside Rg,, Re, Rb, and schisantherin A concentration.关键词
人参皂苷Rg1/人参皂苷Re/人参皂苷Rb1/五味子醇甲/LC-MS/MS联用仪Key words
ginsenoside Rg,/ ginsenoside Re/ ginsenoside Rb,/ schisantherin A/LC-MS/MS分类
医药卫生引用本文复制引用
夏素霞,李国信,姜春梅,唐思,张士良,王月敏,杨瑞..液相色谱-质谱联用法同时测定生脉注射液中人参皂苷Rg1、Re、Rb1和五味子醇甲在健康人血浆的浓度[J].中国临床药理学杂志,2011,27(10):789-792,4.基金项目
辽宁省教育厅基金资助项目(20060549) （20060549）
