陕西医学杂志2011，Vol.40Issue(9)：1107-1111,5.
年龄相关听力损失小鼠耳蜗毛细胞的凋亡抑制剂治疗研究
Mechanism of apoptosis of hair cell in the cochlea of age-related hearing loss
摘要
Abstract
Objective: Age-related hearing loss (Presbycusis) is the most common type of sensory impairment in human being. However, the molecular mechanisms of the presbycusis remain unclear. Apoptosis of outer hair cells (OHCs) in the cochlea has been found in aging animals. Methods: In this study,we investigated the apoptosis of hair cell in the cochlea of age-related hearing loss generated by ENU mutagenesis. Results: The results showed that the nmf308 mice with progressive hair cell loss along a base to apex gradientwith age-related hearing loss. The cochlear OHCs reduced from 5-10% at 1 month to 100% at 3 month in the basal region. Substantial amounts of TUNEL-postive OHCs nuclei appeared at 1 month age. And activated caspase-3 labeling demonstrated that most OHCs appeared at 2 months age. Conclusion; These result suggest that DNA single strand break is attributed primarily to apoptosis of cochlear lesion, whereas the later stage of lesion expansion leads to activation of caspase-3 activity reduced with further progression of nuclear condensation in age-related hearing loss.关键词
听力受损者/病理生理学/年龄因素/毛细胞/细胞凋亡/耳蜗/模型,动物/小鼠Key words
Hearing impaired persons/physiopathology/Age factors/Hair cell/Apoptosis Cochhea/Models,animal/Mice分类
医药卫生引用本文复制引用
张正民,李胜利..年龄相关听力损失小鼠耳蜗毛细胞的凋亡抑制剂治疗研究[J].陕西医学杂志,2011,40(9):1107-1111,5.基金项目
国家自然科学基金资助对外交流与合作项目(No 30210103151) （No 30210103151）
美国NIH项目(No R01DC007392) （No R01DC007392）
陕西省科技攻关项目(No 2004K16-G5) （No 2004K16-G5）
