化工进展2011，Vol.30Issue(7)：1562-1569,8.
醇脱氢酶不对称还原制备手性醇的研究进展
Progress of asymmetric reduction with alcohol dehydrogenase for preparation of chiral alcohols
摘要
Abstract
For preparative applications in asymmetric reduction with alcohol dehydrogenase, three issues have to be considered: An appropriate enzyme, an efficient coenzyme-regenerating method and a suitable in vitro evolution technique. Enzyme is the source of effective biocatalyst. The native or recombinational alcohol dehydrogenases from Rhodococcus sp., Lactobacillus sp., Leifsonia sp. And thermophilic microbiology, and their catalysis were analyzed. The advantages and disadvantages of coenzyme-regeneration methods were discussed. The efficient coenzyme-regeneration can decrease costs for large-scale application. In vitro evolution technique can improve the activity, stability and selectivity of target enzyme. The final aim is to establish effective and green ways to obtain chiral drug intermediates by enzymatic synthesis.关键词
醇脱氢酶；手性中间体；辅酶再生；体外进化Key words
alcohol dehydrogenase/ chiral Intermediates/ coenzyme regeneration/ in vitro evolution分类
生物科学引用本文复制引用
谌容,王秋岩,殷晓浦,魏东芝,谢恬..醇脱氢酶不对称还原制备手性醇的研究进展[J].化工进展,2011,30(7):1562-1569,8.基金项目
国家自然科学基金(21006018)及浙江省科技厅(2009C31086)及杭州师范大学中青年培育基金(2010QNl9)项目。 （21006018）
