中国美容医学2011，Vol.20Issue(7)：1100-1102,3.
低氧对人脐带间充质干细胞成脂肪分化的影响
Effect of hypoxia on the adipogenic defferentiation potential of human umbilical cord mesenchymal stem cells
摘要
Abstract
Objective To investigate the effect of hypoxia on the adipogenic defferentiation potential of human umbilical cord mesenchymal stem cells (hUCMSCs),and provide experimental evidence for selecting seeded cells of adipose tissue engineering. Methods The cultural conditions includes hypoxia (2%O2), normoxia (20%O2), 10% FBS growth medium (GM), 10% FBS adipogenic defferentiation medium (DM);hUCMSCs were cultured in vitro and divided into three groups according to the cultural conditions: control group (normoxia + GM 5d,normoxia + DM 2 weeks), experimental group 1(hypoxia + GM 5d,hypoxia + DM 2 weeks),experimental group 2(hypoxia + GM 5d,normoxia + DM 2 weeks). After 2 weeks of culture.the three groups were stained by oil red 0. Results Compared with control group, the number of the fat-like cells decreased obvously in experimental group 1,but the number of the fat-like cells increased significantly in experimental group 2. Conclusions hUCMSCs pre-exposed to hypoxia when switched to normoxia exhibited significantly higher adipogenic defferentiation capacity.关键词
低氧；人脐带间充质干细胞；分化Key words
hypoxia/human umbilical cord mesenchymal stem cells/ defferentiation分类
生物科学引用本文复制引用
唐军,徐斌,刘毅..低氧对人脐带间充质干细胞成脂肪分化的影响 [J].中国美容医学,2011,20(7):1100-1102,3.基金项目
国家自然科学基金(30872689) （30872689）
