临床与实验病理学杂志2011，Vol.27Issue(11)：1185-1188,4.
鼠神经干细胞对胶质瘤C6细胞侵袭能力的影响
Impact on invasiveness of C6 glioma cell by the action of neural stem cells of rat
摘要
Abstract
Purpose To explore the impact and mechanism of neural stem cells of rat on the invasiveness of C6 glioma cells of rat. Methods The cerebral cortices of newborn 1 -2 day Sprague-Dawley rats ( SD rats ) were extracted, then the neural stem cells in se-rumfree medium were separated and cultured. These cells were stained with NSE immunohistochemical staining. After that the neural stem cells were co-cultured with C6 glioma cell of rat in vitro. The invasiveness of neural stem cells of rat which is co-cultured with C6 glioma cell of rat ( the experimental group ) and individually cultured C6 glioma cell of rat ( the control group ) were detected by utilizing 2D &3D growth experiments and Transwell experiments respectively. Result In two-dimensional growth experiment, the average diameter of C6 cells in control group was ( 18. 53 ± 1. 32 ) μm, however, in experimental group it was ( 13. 44 ± 1. 45 ) μm. The difference has statistical significance( P <0. 01 ). In three-dimensional growth experiment, the average diameter of C6 cells in control group was( 20. 34 ± 1. 5 )μm, however,in experimental group it was ( 15. 52 ± 1. 43 ) μm. The difference has statistical significance ( P <0. 01 ). In the Transwell invasion experiment, the average number of membrane-spanning cells was ( 36. 45 ± 1. 36 ) in control group compared with ( 22. 73 ± 1. 66 ) in experimental group . The difference has statistical significance( P <0. 01 ). Conclusion After co-culturing with neural stem cells, the invasiveness of C6 cells decreases obviously.关键词
胶质瘤/神经干细胞/C6细胞/侵袭Key words
glioma/neural stem cells/C6 cells/invasion分类
医药卫生引用本文复制引用
刘楠,杨俊荣,宋月佳,佟志国,宋菡姝,戚基萍..鼠神经干细胞对胶质瘤C6细胞侵袭能力的影响[J].临床与实验病理学杂志,2011,27(11):1185-1188,4.基金项目
黑龙江省教育厅科学技术研究项目(12511174) （12511174）
