南方医科大学学报2011，Vol.31Issue(10)：1748-1752,5.DOI:44-1627/R.20111004.1115.007
新生血管可视化转基因小鼠的建立
Establishment of transgenic mice with visualized neovascularization
摘要
Abstract
Objective To establish transgenic mice with GFP expression in the vascular endothelium during neovascularization. Methods The vector nestin-hsp68-gfp containing nestin second inrron was introduced into U251 cells and the expression level of GFP was detected by fluorescence microscopy. Transgenic mice were produced by microinjection. The genome of the offspring mice was screened by PCR, and GFP expression in the vascular endothelium was detected using immunohistochemistry. Results Thirteen offspring mice were obtained and 2 of them were positive for GFP in the vascular endothelium as detected by PCR. GFP was detected in the offspring mice both at the embryonic stage and after birth. Conclusion The transgenic mice with GFP expression in the vascular endothelium during neovascularization have been successfully established.关键词
巢蛋白/新生血管/转基因小鼠/绿色荧光蛋白Key words
nestin/ neovascularization/ transgenic mice/ green fluorescent protein分类
医药卫生引用本文复制引用
李振林,高毅,贾俊双,陈宋钦,王海红,安靓..新生血管可视化转基因小鼠的建立[J].南方医科大学学报,2011,31(10):1748-1752,5.基金项目
广东省自然科学基金(8151051501000005) （8151051501000005）
