河南农业科学2011，Vol.40Issue(6)：115-117,121,4.
蝴蝶兰ACC氧化酶和ACC合成酶融合反义表达载体的构建
Construction of Double-antisense Expression Vector of ACC Oxidase and ACC Synthase Gene in Phalaenopsis
摘要
Abstract
According to the reported ACC oxidase (1-aminocyclopropane-l-carboxylate oxidase, ACO) gene and ACC synthase( ACS) gene cDNA sequences, two pairs of specific primers were designed and synthesized,which were obtained from total RNA of Phalaenopsis by RT-PCR. The cDNA fragments were linked into a plant binary vector pBI221 between the CaMV 35S promoter and nos terminator. Then the recombinant fragment including ACC, ACO and 35S promoter was cloned into plant expression vector pCAMBIA3301 in orientation. The positive clones were detected by PCR technique and analyzed by the restriction enzyme. The constructs were transferred into agrobacterium LBA4404 via frozen-fusion method and had been used to transform Phalaenopsis protocorm by agrobacteria.关键词
蝴蝶兰；ACC氧化酶；ACC合成酶；融合反义基因；根癌农杆菌Key words
Phalaenopsis hybrid / ACC oxidase gene/ ACC synthase gene/ Double-antisense RNA/ Agrobacterium tume faciens分类
农业科技引用本文复制引用
王宇腾,崔波,蒋素华,武思,牛苏燕,叶永忠..蝴蝶兰ACC氧化酶和ACC合成酶融合反义表达载体的构建[J].河南农业科学,2011,40(6):115-117,121,4.基金项目
河南省科技攻关项目(0524050005) （0524050005）
