中国药理学通报2011，Vol.27Issue(12)：1723-1727,5.DOI:10.3969/j.issn.1001-1978.2011.12.022
吡那地尔、美托洛尔、谷氨酰胺、胰岛素单独及联合使用对H9c2心肌细胞缺氧/复氧损伤的抗凋亡作用及机制
Antiapoptotic effects and mechanism of pinacidil, metoprolol,glutamine, insulin single and combination on H9c2 cardiac myocytes exposed to simulated hypoxia/reoxygenation
摘要
Abstract
Aim To investigate protective effects and mechanism of pinacidil, metoprolol, glutamine, insulin on H9c2 cardiac myocytes exposed to simulated hy-poxia/reoxygenation. Methods H9c2 cardiac myocytes were randomly divided into seven groups: ①Control group; ② Hypoxia/reoxygenation ( H/R ) group; ③ Pinacidil ( Pin ) group; ④ Metoprolol ( Met ) group; ⑤Glutamine ( Glu ) group; ⑥ Insulin ( Ins ) group; ⑦ ) Pinacidil + Metoprolol + Glutamine + Insulin ( PMGI ) group. The survival rates of cardiomyocytes were detected. The activity of lactate dehydrogenase ( LDH ) in the culture medium was measured. The mitochondria were labeled by Rhodamine123 fluorescence probe and the fluorescent intensity produced by Rhl23 was measured, which reflected changes of mitochondria! Mem-brane potential ( MMP, ΔΨm ). The apoptotic percentage was measured with flow cytometry. Results The combined use of pinacidil, metoprolol, glutamine, and insulin could protect the H9c2 cardiac cell membrane caused by hypoxia/reoxygenation injury, increase cell survival rate,decrease LDH leakage, decrease the apoptotic rate and increase the mitochondria! Membrane potential. Conclusions Compared to the individual drug, the combination of the different mechanisms protects the mitochondria of cardiac myocyte, decreases the injury and enhances antiapoptotic effection of myocar-dial cells.关键词
缺氧/复氧损伤/联合用药/心肌细胞存活率/乳酸脱氢酶/细胞凋亡率/线粒体膜电位Key words
hypoxia/reoxygenation/ drug combination/ survival rate of cardiomyocytes/ lactate dehydrogenase/ apoptotic percentage/ mitochondrial membrane potential分类
医药卫生引用本文复制引用
孙畅,臧婵媛,吴艳娜,温克,康毅,娄建石..吡那地尔、美托洛尔、谷氨酰胺、胰岛素单独及联合使用对H9c2心肌细胞缺氧/复氧损伤的抗凋亡作用及机制[J].中国药理学通报,2011,27(12):1723-1727,5.基金项目
高等学校博士学科点专项科研基金(No 20070062009) （No 20070062009）
