实用医学杂志2011，Vol.27Issue(18)：3284-3287,4.DOI:10.3969/j.issn.1006-5725.2011.18.009
氟伐他汀对大鼠肾缺血再灌注损伤P27和PCNA表达的影响
Effect of fluvastatin on expression of P27 and PCNA in renal Ischemia/reperfusion model of rats
摘要
Abstract
Objective To investigate the expressions of P27 and PCNA on renal tubular epithelial cells in renal ischaemia and reperfusion injury,and evaluate the effect and possible mechanism of HMG-COA reductase inhibiter fluvastatin on expressions of P27 and PCNA in the cells. Methods 30 rats were randomly divided into 3 groups: sham-operated group(n = 10); IRI group (n = 10); fluvastatin treatment group (n = 10). The in vivo study was conducted by establishing rat model of acute ischemic renal failure through ischaemia/reperfusion and sacrificing the rats 24 hours after both renal hila were clamped with small vascular clamps for 60 minutes and released. At 24 hours after reflow, renal morphology was guantified. Also, the expression and localization of P27 and PCNA were examined in kidney sections by immunohistochemistry. Results Our date shown that the extend of renal morphology changes in Fluvastatin treatment group were remarkably less than that in IRI group , Cell Proliferation Index's (PI) in IRI group was the highest among three groups, and significantly higher than that in Fluvastatin treatment group. The immunohistochemistry analysis shown that the expression of P27 was significantly lower than that in sham group , and elevated by Fluvastatin treatment (P<0.01). The level of P27 had negative correlation with PCNA (r = -0.609 ,P< 0.01). Paller's grades had positive correlation with PI (r = 0.872,P < 0.01 )and negative correlation with P27 (r = - 0.713 ,P < 0.01). Conclusion The level of P27 expression decreased and PCNA expression increased at 24 hours after reflow in the rat model of acute ischemic renal failure. Fluvastatin playing a role in renal protection may enhance P27 expression, make cell cycle arrest in Gl phase and help for DNA repairing.关键词
氟伐他汀/缺血再灌注/P27/PCNA/肾小管上皮细胞Key words
Fluvastatin/Ischaemia reperfusion/P27/PCNA/Renal tubular epithelial cells引用本文复制引用
田蕾,孙洞箫..氟伐他汀对大鼠肾缺血再灌注损伤P27和PCNA表达的影响[J].实用医学杂志,2011,27(18):3284-3287,4.基金项目
辽宁省教育厅科研项目(编号:L2010308) （编号:L2010308）
