中国组织工程研究与临床康复2011，Vol.15Issue(45)：8391-8394,4.DOI:10.3969/j.issn.1673-8225.2011.45.007
兔脂肪干细胞在含有转化因子和转铁蛋白培养基中向软骨细胞的分化
Chondrogenic differentiation of rabbit adipose-derived stem cells in culture medium containing transforming factors and transferrin
摘要
Abstract
BACKGROUND: The choice of seed cells is a key factor to the cartilage tissue engineering.OBJECTIVE: To explore the chondrogenic potential of adipose-derived stem cells (ADSCs) in culture medium containingtransforming factors and transferring.METHODS: ADSCs were obtained from the subcutaneous adipose tissue of 6-month-old New Zealand white rabbits'neckbackby mechanical digestion and enzyme digestion, and then cultured and amplified in vitro. The adhesion and growth of cells wereobserved using inverted phase contrast microscope. Two weeks after the cells were cultured in chondrogenic medium, collagentype II expression was detected by immunohistochemistry staining.RESULTS AND CONCLUSION: The primary stem cells isolated from adipose tissue adhered on the plate in 24 hours, andreached 90% confluence in single layers after 96 hours. The cartilage nodules were observed at 7 days after ADSCs werecultured under chondrogenic medium, while collagen type II was positive 14 days later. ADSCs of rabbits can expresschondrogenic phenotype when they were cultured in chondrogenic medium, which proves the ADSCs can be one of the optionsfor cartilage tissue engineering.关键词
脂肪干细胞/软骨细胞/软骨组织工程/分化/兔分类
医药卫生引用本文复制引用
杨国庆,王兆杰,安荣泽,赵俊延,齐新文..兔脂肪干细胞在含有转化因子和转铁蛋白培养基中向软骨细胞的分化[J].中国组织工程研究与临床康复,2011,15(45):8391-8394,4.基金项目
贵州省科技厅资助项目(黔科通NY字[2006]62-2) （黔科通NY字[2006]62-2）
珠海市医学重点专科建设基金资助(珠卫[2008]80). （珠卫[2008]80）
