中国农业科学2011，Vol.44Issue(12)：2495-2503,9.DOI:10.3864/j.issn.0578-1752.2011.12.011
24-表油菜素内酯对低氧胁迫下黄瓜幼苗碳水化合物代谢的影响
Effects of 24-Epibrassinolide on Carbohydrate Metabolism and Enhancement of Tolerance to Root-Zone Hypoxia in Cucumber(Cucumis sativus L.)
摘要
Abstract
[Objective] The aim of the work was to study the effects of 24-epibrassinolide (EBR) added to nutrient solution on carbohydrate metabolism of cucumber {Cucumis sativus L.) under root-zone hypoxia. [Method] Seedlings of a relatively hypoxia-resistant cultivar, 'L(u)bachun No.4', and a relatively hypoxia-sensitive cultivar, 'Zhongnong No.8', were hydroponically grown for 8 days in normoxic and hypoxic nutrient solutions with and without addition of EBR at 1 μg L-1. And the effects of EBR on contents of carbohydrates in leaves and roots and activities of glycolytic enzymes in the roots were investigated. [Result] Application of EBR to normoxic nutrient solution promoted the accumulation of total soluble sugars in leaves and roots, but had no effects on activities of glycolytic enzymes in the roots. Changes in contents of starch and glucose, and activities of acid invertase and ATP-dependent phosphofructokinase enzymes in the roots showed obviously different between two genotypes cucumber in response to root-zone hypoxia. EBR added to hypoxic nutrient solution caused an increase in the concentrations of fructose and sucrose and activities of major glycolytic enzymes in the roots, but exerted little influence on carbohydrate concentrations in the leaves. [Conclusion] These results suggest that EBR added to hypoxic nutrient solution may stimulate the photosynthate allocation down to roots and activities of major glycolytic enzymes, and eventually enhanced tolerance of cucumber plants to root-zone hypoxia.关键词
黄瓜/24-表油菜素内酯/低氧胁迫/碳水化合物/糖酵解Key words
cucumber (Cucumis sativus L.)/24-epibrassinolide/hypoxia stress/carbohydrates/glycolysis引用本文复制引用
康云艳,杨暹,郭世荣,张营营..24-表油菜素内酯对低氧胁迫下黄瓜幼苗碳水化合物代谢的影响[J].中国农业科学,2011,44(12):2495-2503,9.基金项目
国家"973"计划项目(2009CB119000),国家自然科学基金项目(30871763)、农业部现代农业产业技术体系建设专项资金资助项目(CARS25-C-03)、2010年广东高校优秀青年创新人才培养计划(育苗工程) （2009CB119000）
