中国组织工程研究与临床康复2011，Vol.15Issue(45)：8439-8442,4.DOI:10.3969/j.issn.1673-8225.2011.45.017
常氧及低氧条件下胶质源性神经营养因子体外诱导中脑神经干细胞向多巴胺能神经元的分化
Glial-derived neurotrophic factor induces the in vitro differentiation of mesencephalic neural stem cells into dopaminergic neurons under normoxic and hypoxic conditions
摘要
Abstract
BACKGROUND: Directed differentiation and proliferation of nerve stem cells are regulated by their own genes and externalsignals.OBJECTIVE: To observe the differentiation of mesencephalic neural stem cells into dopaminergic neurons under normoxicandhypoxic conditions induced by glial-derived neurotrophic factor (GDNF).METHODS: Under aseptic conditions, we isolated mesencephalic tissues from E12 mouse embryos. Single cell suspension wasprepared by pancreas enzyme digestion and mechanical pipetting, and were cultured and proliferated in serum-free medium.Nestin immunocytochemistry staining was used to identify nerve stem cells. Purified nerve stem cells differentiated in serummedium. Neuron-specific enolase and glial fibrillary acidic protein immunocytochemistry staining methods were performed toidentify neurons and astrocytes, respectively. All the cells were divided into normoxia group, normoxia+GDNF group, hypoxiagroup, hypoxia+GDNF group, and then cultured in serum medium.RESULTS AND CONCLUSION: Under the hypoxia condition, mesencephalic neural stem cells had a higher differentiated ratethan that in the normoxia group. The number of differentiated cells in the hypoxia+GDNF group was higher than that of the othergroups, and the differentiated cells had a mature phenotype. Lt is indicated that under the hypoxic condition, GDNF can extremelypromote the differentiation of nerve stem cells into dopaminergic neurons with good morphology and mature function.关键词
常氧/低氧/胶质源性神经营养因子/中脑神经干细胞/多巴胺能神经元/酪氨酸羟化酶分类
医药卫生引用本文复制引用
罗特坚,丁继固..常氧及低氧条件下胶质源性神经营养因子体外诱导中脑神经干细胞向多巴胺能神经元的分化[J].中国组织工程研究与临床康复,2011,15(45):8439-8442,4.基金项目
湖北省教育厅重点课题(D20092802). （D20092802）
