中国组织工程研究与临床康复2011，Vol.15Issue(45)：8459-8462,4.DOI:10.3969/j.issn.1673-8225.2011.45.022
自体嗅黏膜移植联合β-七叶皂甙钠干预脊髓损伤大鼠基质金属蛋白酶2、9的表达
Effects of autologous olfactory mucoma combined with beta-aesine sodium on expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 in rats following spinal cord injury
摘要
Abstract
BACKGROUND: After spinal cord injury, over-expression of metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) contributes to secondary tissue damage of the spinal cord.OJECTIVE: To study the influence of autologous olfactory mucoma combined with B-aesine sodium on expression of MMP-2,9 and the recovery of neural function of injured spinal cord in adult rats.METHODS: In this study, healthy Sprague-Dawley rats with T10 spinal cord hemisection were divided into four groups as follows: control group (A), autologous olfactory mucoma transplantation group (B), B-aesine sodium group (C), combination group (D). RESULTS AND CONCLUSION: The BBB scales showed that rats in groups B, C and D had more improvement than those in group A at series of phases at 7 and 14 days after operation (P < 0.05), and the BBB score of group D was much higher than that ofgroupBandgroupC(P< 0.05). The expression of MMP-9 and MMP-2 in injured spinal cord increased significantly at all time points. The number of positive cells in group D was less than that in groups B and C at the corresponding time points (P<0.05), but there were no statistically significant differences between group B and C (P > 0.05). The method of treating spinal cord injury by autologous olfactory mucosa transplantation combined with B-aesine sodium has good curative effect, and can largely improve hind limbs motor function, which may be related to inhibiting the overexpression of MMP-9 and MMP-2 genes.关键词
自体嗅黏膜/脊髓损伤/β-七叶皂甙钠/基质金属蛋白酶/大鼠分类
医药卫生引用本文复制引用
程华,刘曾旭,徐年香,罗小凤,余庆,刘卉芳,刘德明..自体嗅黏膜移植联合β-七叶皂甙钠干预脊髓损伤大鼠基质金属蛋白酶2、9的表达[J].中国组织工程研究与临床康复,2011,15(45):8459-8462,4.基金项目
江西省教育厅课题(GJJ09097),江西省科技支撑项目(2009BSB09500). （GJJ09097）
