中国组织工程研究与临床康复2011，Vol.15Issue(45)：8483-8486,4.DOI:10.3969/j.issn.1673-8225.2011.45.027
PCR-SSO流式荧光微珠法HLA-A*02高分辨分型检测模棱两可结果的统计分析
Statistical analysis for ambiguous results of HLA-A*02 high-resolution genotyping by using PCR-SSO flow fluorescent bead method
张容达 1杜娟 2马晓莉 2张伯伟2
作者信息
- 1. 复旦大学物理系生物物理重点实验室,上海市,200433
- 2. 河南省红十字血液中心,河南省郑州市,450012
- 折叠
摘要
Abstract
BACKGROUND: Due to the complexity of the HLA typing system, ambiguous situations occur frequently during HLA typing of the donors.OBJECTIVE: To statistically analyze the ambiguous ratio and combined allele groups of HLA-A*02 high-resolution genotyping by using PCR-SSO flow fluorescent bead method, and to investigate the method to resolve the ambiguous results of HLA-A*02 high-resolution genotyping and improve the ratio of high-resolution genotyping.METHODS: 1100 pieces of HLA genotyping were collected for statistical analysis. We calculated the percentages of those combinations that contained HLA-A*02:01\02:09 ambiguity, given by the software HLA Fusion 2.0; in the other hand, we searched out those alleles which were highly correlated with A*02:09 by comparing these combination percentages with the A*02:09 genetic polymorphism distribution.RESULTS AND CONCLUSION: 279 out of 1039 effective samples (279/1039, 26.853%) were found for the results of having A*02:01/02:09 ambiguities that could be categorized into 19 groups in NM DP code. The most frequently groups were DM DC, DYVK, GMDD, GMDE. Ln another cases, 34 identified as carrying A*02:09 were analyzed appearing that A*02:09 was highly correlated to B*07:02(0.2647), DRB1*01:01 (0.2647), DRB1*03:01 (0.1176). We selected 38 high risky samples and tested their 4-7 exons, and confirmed by SBT, only 1 sample could be identified as A*0209, while other 241 samples lacked of A*02:09. A reasonable judgment based on statistical analysis and necessary review can effectively simplify the experimental procedures, save time, reduce costs, and increase high-resolution ratio.关键词
HLA-A*0201/0209模棱两可/中华骨髓库/PCR-SSO/HLA分型/基因分类
医药卫生引用本文复制引用
张容达,杜娟,马晓莉,张伯伟..PCR-SSO流式荧光微珠法HLA-A*02高分辨分型检测模棱两可结果的统计分析[J].中国组织工程研究与临床康复,2011,15(45):8483-8486,4.
