中国组织工程研究与临床康复2011，Vol.15Issue(45)：8503-8507,5.DOI:10.3969/j.issn.1673-8225.2011.45.032
人脐带间充质干细胞诱导成骨及治疗骨缺损
Human umbilical cord mesenchymal stem cells induce ossification and treat bone defects
曲志国 1野向阳 1林辉 1崔正宏1
作者信息
- 1. 四平市中心人民医院,吉林省四平市,136000
- 折叠
摘要
Abstract
BACKGROUND: Umbilical cord mesenchymal stem cells have greater expansion capacity and low immunogenicity in ossification and tissue and organ repair, and their growth potential into a colony is earlier than mesenchymal stem cells of the bone marrow and other sources.OBJECTIVE: To observe the clinical effect of umbilical cords mesenchymal stem cells on inducing ossification and treating bone defects.METHODS: Human umbilical cord mesenchymal stem cells were isolated by the tissue block culture. In vitroosteogenicability was confirmed by the morphology changes of human umbilical cord mesenchymal stem cells, alizarin red staining, alkaline phosphatase staining, and type I collagen expression. Human umbilical cord mesenchymal stem cells transplantation was performed in patients with bone defects, and conditions of callus growth were regularly reviewed after transplantation. RESULTS AND CONCLUSION: In vitro induction experiments confirmed that umbilical cord mesenchymal stem cells had a clear osteogenic role. Two months after cell transplantation, X-rays showed bony callus formation, fracture interspace vague. Osseous connection formation was found at 3 months after cell transplantation. It was confirmed that umbilical cord mesenchymal stem cells exerted a role of osteogenic induction, and could repair bone defects via in vivo cell transplantation.关键词
人脐带间充质干细胞/干细胞移植/骨不连/骨缺损/诱导分类
医药卫生引用本文复制引用
曲志国,野向阳,林辉,崔正宏..人脐带间充质干细胞诱导成骨及治疗骨缺损[J].中国组织工程研究与临床康复,2011,15(45):8503-8507,5.
