中国组织工程研究与临床康复2011，Vol.15Issue(45)：8542-8546,5.DOI:10.3969/j.issn.1673-8225.2011.45.041
脐带间充质干细胞促进骨愈合的体外实验及临床应用1例
In vitro experiment of umbilical cord mesenchymal stem cells to promote bone healing and clinical application in one case
李华涛 1边琳芬 1吴胜刚 1李强 1王黎丽 1戴育成 2刘洋3
作者信息
- 1. 解放军第九八医院烧伤整形科,浙江省湖州市,313000
- 2. 南昌大学医学院第二附属医院分子医学研究中心,江西省南昌市,330000
- 3. 协和华东干细胞基因工程有限公司,浙江省湖州市,313000
- 折叠
摘要
Abstract
BACKGROUND: Umbilical cord mesenchymal stem cells (UCMSCs) are more original than adult ones. Studies have shown that UCMSCs have higher telomerase activity, shorter culture doubling time and wider lineage differentiation. OBJECTIVE: To explore the potency of UCMSCs differentiated into osteoblasts in vitro and their clinical effects on bone nonunion. METHODS: UCMSCs were isolated from Wharton's jelly of human umbilical cord; after serial subculture in vitro, MSCs were tested for their immunophenotype and osteogenic differentiation. Clinical application of UCMSCs transplantation was also performed to a patient with refractory exposed bone fracture evoked by infection.RESULTS AND CONCLUSION: UCMSCs exhibited fibroblastic morphology and long-term stable culture. These passaged cells presented MSCs immunophenotype and there were a great amount of calcium deposition and nodes in intracytoplasm detected by alizarin red and Von Kossa staining, respectively, after osteogenicdifferentiation. The patient was treated with UCMSCs suspension for four times, granulation tissue grew rapidly to fulfill the sinus tract and became epithelized. Twelve days after treatment, the fracture was healed. It indicates that UCMSCs have self-renewal and pluripotent differentiation potential and can differentiate into osteoblasts in vitro. For treatment of bone nonunion, UCMSCs has the ability to improve microenvironment.关键词
脐带间充质干细胞/移植/成骨细胞分化/骨折外露/骨愈合分类
基础医学引用本文复制引用
李华涛,边琳芬,吴胜刚,李强,王黎丽,戴育成,刘洋..脐带间充质干细胞促进骨愈合的体外实验及临床应用1例[J].中国组织工程研究与临床康复,2011,15(45):8542-8546,5.
