摘要
Abstract
AIM To develop a method for the determination of amtolmetin guacil (MED-15) and its active metabolite MED-5 and tolmetin in rat plasma by HPLC method. METHODS Plasma samples were extracted with ace-tonitrile. The separation was carried out by using a Hypersil ODS C18 column(250 mm x 4.6 mm,5 μm) with a mobile phase of methanol- 2% glacial acetic acid (60:40, V/V) at a flow rate of 1.0 mL·min-1 ,and detection wave length was set at 313 ran. RESULTS The linear ranges for MED-15, MED-5 and tolmetin were 0.050 - 5.6 mg· L-1, 0.042-5.4 mg·L-1 and 0.039-5.0 mg·L-1(r = 0.995 1, r = 0. 995 7, r = 0.999 0), respectively. The limit of quantitation of MED-15, MED-5 and tolmetin was 0.05,0.042 and 0.039 mg·L-1 .respectively.The relative recoveries of MED-15, MED-5 and tolmetin were 100.3%, 99.4% and 99. 9%, respectively. The absolute recoveries were 88.9% ,87.8% and 88.7% .respectively. The intra-day RSD and inter-day RSD were both less than 12%. CONCLUSION The developed method for the quantification of MED-15, MED-5 and tolmetin in rat plasma is sensitive,accurate, precise and stable,and is suitable for the study on the pharmacokinetics of MED-15 in rats.关键词
呱氨托美丁；MED-5；托美丁；高效液相色谱法Key words
amtolmetin guacil/MED-5/tolmetin/HPLC
