中国临床药学杂志2011，Vol.20Issue(4)：228-231,4.
HPLC法测定人尿液中尼非卡兰浓度
Determination of the concentration of nifekalant in human urine by HPLC
隋因 1唐云彪 1史国兵 1刘倩2
作者信息
- 1. 沈阳军区总医院药剂科，沈阳110016
- 2. 沈阳药科大学药学院，沈阳110016
- 折叠
摘要
Abstract
AIM To develop a HPLC method for the determination of the concentration of nifekalant in human urine and to study its cumulative excretion amounts from urine in Chinese healthy volunteers. METHODS Chromatogra-phy was carried out on μ-Bondapak C18 column (150 mm×4.6 mm, 10 fan), using the mobile phase of 0.05 mol·L-1 NH4H2PO4(pH was adjusted to 5.6 with 25% ammonium hydrous)-methanol-acetonitrile (60 : 20:20, V/V/V) at a flow rate of 1.0 mL·min-1. The UV detection wavelength was set at 270 ran, and the internal standard was carbamazepine. Sixteen qualified healthy volunteers were involved in this study. After two intravenous bolus injections and a continuous intravenous injection of nifekalant within 6 h, urine samples at different intervals were collected. RESULTS The urine standard curve was linear within the range of 20 - 5 000 μg·L-1 with the correlation coefficient more than 0.996. The lower limit of quantification (LLOQ) was 20 μg·L-1. The precisions (RSD) of intra-day and inter-day at low, medium and high concentrations (QCs) were less than 10.9%. The average recoveries of nifekalant at low, medium and high concentrations (QCs) were( 83.0± 18.9)%,(85.0±26.2)% and (74.4±4.8)%, respectively. CONCLUSIONThe established method is simple, accurate and specific. It has been successfully used for the analysis of nifekalant inurine.关键词
尼非卡兰；HPLC；累积排泄量Key words
nifekalant/HPLC/cumulative excretion amount引用本文复制引用
隋因,唐云彪,史国兵,刘倩..HPLC法测定人尿液中尼非卡兰浓度[J].中国临床药学杂志,2011,20(4):228-231,4.
