南方医科大学学报2011，Vol.31Issue(11)：1944-1948,5.DOI:44-1627/R.20111025.0915.001
Survivin基因沉默抑制胃癌MGC-803细胞增殖并增强其对塞来昔布的敏感性
Survivin gene silencing inhibits the proliferation of human gastric cancer MGC-803 cells and enhances their sensitivity to celecoxib
方雷 1吴海滨 2陈晓岗1
作者信息
- 1. 宁波市第一医院普通外科,浙江宁波315010
- 2. 宁波大学医学院,浙江宁波315211
- 折叠
摘要
Abstract
Objective To investigate the effect of small interfering RNA (siRNA)-mediated survivin knock-down on the proliferation of human gastric cancer MGC-803 cells and their sensitivity to celecoxib. Methods The siRNA against survivin was constructed and transfected into MGC-803 cells via LipofectamineTM 2000. The expression of survivin in the transfected cells was detected by RT-PCR and Western blot, and flow cytometry was used to detect the cell cycle changes. The sensitivity of the cells to celecoxib after transfection was examined using MTT assay and clonogenic assay. Results The protein and mRNA levels of survivin in MGC-803 cells were decreased significantly after siRNA transfection, which also caused cell cycle arrest in G0/G1, phase. The sensitivity of MGC-803 cells to celecoxib was significantly increased after siRNA transfection. Conclusion siRNA-mediated survivin silencing causes growth suppression of MGC-803 cells and enhances their sensitivity to celecoxib in vitro.关键词
Survivin/siRNA/胃癌/MGC-803/细胞增殖/塞来昔布Key words
survivin/ small interfering siRNA/ gastric cancer/ MGC-803/ cell proliferation/ celecoxib分类
医药卫生引用本文复制引用
方雷,吴海滨,陈晓岗..Survivin基因沉默抑制胃癌MGC-803细胞增殖并增强其对塞来昔布的敏感性[J].南方医科大学学报,2011,31(11):1944-1948,5.
