中国农业科学2011，Vol.44Issue(13)：2796-2807,12.DOI:10.3864/j.issn.0578-1752,2011.13.018
崂山奶山羊分子系谱的构建及家系遗传特征分析
Construction of Molecular Genealogy and Analysis of Family Genetic Characteristics in Laoshan Dairy Goat
摘要
Abstract
[Objective] The molecular genealogy of population known pedigree records in Laoshan Dairy Goat was constructed by using microsatellite DNA markers technology to verify the consistency with the known pedigree records, identify the factors affecting paternity testing, ascertain the genetic characteristics of family and provide a scientific basis for the conservation and genetic management of the groups. [Method] Twelve microsatellite markers with high polymorphism were selected from 25 ones for 212 Laoshan Dairy Goat with known pedigree records, parentage were analyzed with Cervus vs 2.0, molecular genealogy was built with Pedigraph vs 2.2, the genetic characteristics and phylogenesis of family were analysed with Molkin vs 3.0, MEGA4 and GeneClass2. The correlation coefficient between the exclusion probability(EP) per locus and PIC, He, Ho, K was analyzed with SPSS vs 16, and the effect of different locus and information source on the accuracy of paternity testing was analysed, also. [Result] Twelve highly polymorphic microsatellite DNA markers were selected from 25 ones in Laoshan Dairy Goat, the mean PIC value is 0.687, the mean number of allele is 5.75, the mean expected heterozygosity is 0.730. The two-parents combined exclusion probability is 0.9998 on the confidence level of 95%. The biological mother or grandmother of 115 offsprings were found from 80 candidate parents, 3 individuals were not accorded with the known pedigree records, the conincidence rate is 98.58%. The molecular genealogy was constructed on the basis of paternal strains. The correlation coefficient between the exclusion probability and the PIC is the biggest (0.99), and K with the smallest correlation coefficient (0.67). The CEP2 is more than 99.73% with 8 microsatellite loci. [Conclusion] Twelve microsatellite loci can be used for analysis of paternity or construct molecular genealogy in Laoshan Dairy Goat group. The built families have a good genetic basis, and a high genetic diversity within each family or among families.关键词
微卫星/奶山羊/亲权鉴定/分子系谱/遗传特征Key words
microsatellite/ dairy goat/ paternity testing/ molecular genealogy/ genetic characteristics引用本文复制引用
纪志宾,王桂芝,王金凤,陈珊珊,王勇,赵金山,程明,王建民..崂山奶山羊分子系谱的构建及家系遗传特征分析[J].中国农业科学,2011,44(13):2796-2807,12.基金项目
国家转基因生物新品种培育科技重大专项(2009ZX08008-004B)、国家公益性行业(农业)科研专项(201103038) （2009ZX08008-004B）
